Guiando o processo de reparo ósseo com a utilização de composto orgânico polivitamínico / Guiding the bone repair process using a multivitamin organic substance

Várias citocinas estão presentes durante o processo inflamatório/reparativo, sendo muitas delas também encontradas na organogênese. O objetivo desse estudo foi avaliar a possibilidade de aproveitar as condições iniciais do processo de reparo e dirigir as citocinas para a formação de um tecido organizado. Para esse estudo foram utilizados sete cães sem raça definida, nos quais foram instalados 56 implantes, sendo oito em cada animal (dois em cada tíbia e dois em cada hemimandíbula). Os grupos foram elaborados da seguinte maneira: grupo controle (um animal), onde foram somente inseridos os implantes, com o preparo do leito de forma convencional; grupo experimental 1 (Exp1), onde foram inseridos os implantes em leitos preparados com a fresagem até 4,5 mm de diâmetro e o espaço preenchido pelo material polimérico; grupo experimental 2 (Exp2), onde foram inseridos os implantes em leitos preparados com a fresagem até 4,5 mm de diâmetro e o espaço preenchido pelo material polimérico acrescido do composto orgânico, em proporção de 30% do peso do polímero (três animais). Os resultados indicaram um aumento da neoformação óssea local e um incremento na velocidade da deposição óssea, verificada principalmente na região supracortical, nos animais do grupo Exp2, diferentemente do grupo Exp1, onde não houve a neoformação óssea e os implantes não apresentaram osseointegração. Concluiu-se, com base nos resultados, que houve produção endógena de BMPs pelas células locais que ficaram isoladas, sugerindo que uma composição de elementos orgânicos e inorgânicos pode direcionar o tecido lesado para uma neoformação óssea organizada.

Several cytokines are present during the inflammatory/repair process, many of which are also found in organogenesis. The aim of this study was to evaluate the possibility to take the initial conditions of the repair process and guide cytokines to form an organized tissue. Seven mongrel dogs were used in this study (56 implants installed, 8 in each animal, being two in each tibia and two in each hemi-mandible). Groups were prepared as follows: Control group (one animal), with the implants inserted using a conventional preparation technique; Experimental group 1 (Exp1), with the implants inserted into sites prepared with a 4.5 mm-drill and the space filled by polymeric material; Experimental group 2 (Exp2), with the same technique in Exp1 plus the organic compound (30% by weight, three animals). The results indicated an increase in local bone formation and an increase in the speed of bone deposition, seen mainly at the cortex of group Exp2. No new bone formation and osseointegration were seen in Group Exp1. It was concluded that there was an isolated, endogenous production of BMPs by local cells suggesting that a composition of organic and inorganic elements can direct the injured tissue to an organized bone formation.

Identification of the nuclear factor kappa-beta (NF-kB) in cortical of miceWistar using Technovit 7200 VCR®

Objective: this study aimed to develop a nondecalcified bone sample processing technique enabling immunohistochemical labeling of proteins by kappa-beta nuclear factor (NF-kB) utilizing the Technovit 7200 VCR® in adultmale Wistar rats.Study Method: A 1.8 mm diameter defect was performed 0.5mm from the femur proximal joint by means of around bur. Experimental groups were divided according to fixing solution prior to histologic processing: Group1- ethanol 70%; Group 2-10% buffered formalin; and Group 3- Glycerol diluted in 70% ethanol at a 70/30 ratio+ 10% buffered formalin. The post-surgical periods ranged from 01 to 24 hours. Control groups included a nonsurgical procedure group (NSPG) and surgical procedures where bone exposure was performed (SPBE) without drilling. Prostate carcinoma was the positive control (PC) and samples subjected to incomplete immunohistochemistry protocol were the negative control (NC). Following euthanization, all samples were kept at 4oC for 7days, and were dehydrated in a series of alcohols at -20oC. The polymer embedding procedure was performed atethanol/polymer ratios of 70%-30%, 50%-50%, 30%-70%, 100%, and 100% for 72 hours at -20oC. Polymerization followed the manufacturer’s recommendation. The samples were grounded and polished to 10-15ìm thickness,and were deacrylated. The sections were rehydrated and were submitted to the primary polyclonal antibody anti-NF-kB on a 1:75 dilution for 12 hours at room temperature.Results: Microscopy showed that the Group 2 presented positive reaction to NF-kB, diffuse reactions for NSPGand SPBE, and no reaction for the NC group.Conclusion: The results obtained support the feasibility of the developed immunohistochemistry technique (AU)